논문검색
Author Sang Cheul Oh, M.D.1,2, Young Do Yoo, Ph.D.1,2, So Young Yoon, M.D.1,2, Seok Jin Kim, M.D.1,2, Jae Hong Seo, M.D.1,2, Kwang Taek Kim, M.D.2,3, Sang Won Shin, M.D.1,2, Yo Han Kim, M.D.5, Yeul Hong Kim, M.D.1,2,4 and Jun Suk Kim, M.D.1,2,4
Place of duty Department of Internal Medicine, 2Genomic Research Center for Lung and Breast/Ovarian Cancers, 3Department of Chest Surgery, 4Brain Korea 21 Project for Medical Science, Korea University College of Medicine, Seoul, Korea, 5Department of Chest Surgery, Konkuk University College of Medicine, Chungju, Korea
Title Microsatellite Alterations in Serum DNA of Lung Cancer Patients
Publicationinfo Cancer Research and Treatment 2003 Aug; 035(04): 289-293.
Key_word Serum DNA, Lung neoplasms, Loss of heterozygosity
Full-Text
Abstract Purpose: Neoplastic progression is accompanied by multiple genetic alterations, which include the functional loss of tumor suppressor genes, and tumor specific genetic alterations are increasingly being investigated as molecular tumor markers. Recently, genetic changes including microsatellite alterations have been found in the serum DNA of cancer patients. Many studies have shown that alterations in the DNA isolated from serum tend to be identical to those found in tumor tissue. If so, serum DNA alterations may be very useful for detecting tumoral genetic changes. The aim of this study was to detect chromosome 3p microsatellite alterations, such as loss of heterozygosity (LOH) and microsatellite instability, in the serum DNA of lung cancer patients.D3S4597, D3S1573). Results: We found that heterozygotes were present in 18 of 46 cases (39%) for D3S4623, and LOH was detected in two of these 18 cases (11%). In D3S4597, heterozygotes were present in 26 of 46 cases (56.5%) and LOH was detected in 9 of these 26 heterozygotes (34.6%). Heterozygotes of D3S1573 were present in 19 of 46 cases (41.3%) and LOH was detected in 7 of these 19 cases (36.8%). The serum DNA of 10 of 26 patients exhibited LOH in at least one of the three markers investigated (38.46%). Conclusion: Our result suggest that the microsatellite alterations in tumor DNA can be detected in the serum of lung cancer patients, and that serum DNA may be usefully used for the diagnosis and screening of lung cancer.(Cancer Research and Treatment 2003;35:289-294